Transformation and mobilization of cloning vectors in Acinetobacter spp.

نویسندگان

  • J T Singer
  • J J van Tuijl
  • W R Finnerty
چکیده

R300B-, RSF1010-, and RK2-derived plasmids were introduced into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413 by transformation and conjugal mobilization. The transformation frequencies of BD413 were 4.2 X 10(6) to 6.3 X 10(6) transformants per micrograms of DNA per 10(9) recipient cells. Conjugal mobilization frequencies were 1.1 X 10(-1) to 8.5 X 10(-1) per recipient. An improved method for the transformation of A. calcoaceticus BD413 is reported.

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عنوان ژورنال:
  • Journal of bacteriology

دوره 165 1  شماره 

صفحات  -

تاریخ انتشار 1986